Cas9(C) — PlasmidDB

Feature Information

Product

C-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)

Type

CDS

Protein Sequence

833 amino acids

KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

Found in 4 Plasmids

Plasmid	Species	Position	Strand
pET SUMO NL-FKBP-Cas9 162730	Homo sapiens	4018..6517	Fwd
KM-CashGem-Xyl2-URA3-LINEAR 174838	Synthetic	6396..8781	Fwd
pIW447-CRISPR XYL2 98906	Synthetic	2340..4725	Fwd
pIW601-KmCRISPR 98907	Synthetic	2275..4660	Fwd

DNA Sequence Variants

Sequence context may differ across plasmids due to position and neighboring features.

pET SUMO NL-FKBP-Cas9 162730

4018..6517 (Fwd)

DNA Sequence (2499 bp)

AAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGAC

Protein Sequence (833 aa)

KM-CashGem-Xyl2-URA3-LINEAR 174838

6396..8781 (Fwd)

DNA Sequence (2385 bp)

TGTTTCGACTCTGTCGAAATTTCTGGTGTCGAAGACAGATTCAATGCTTCTTTGGGTACTTACCACGATTTGTTGAAAATCATTAAGGATAAGGATTTCTTAGATAACGAGGAGAACGAAGATATTTTGGAGGATATTGTCTTGACTTTGACTTTGTTTGAGGATAGAGAAATGATCGAGGAAAGATTGAAGACTTACGCTCATTTGTTCGATGATAAGGTCATGAAGCAATTGAAGAGAAGAAGATACACTGGTTGGGGTAGATTGTCCAGAAAGTTGATCAACGGTATTAGAGATAAGCAATCCGGTAAGACCATTTTGGATTTCTTGAAGTCTGACGGTTTCGCTAATAGAAACTTTATGCAATTGATTCACGACGATTCTTTGACTTTTAAGGAAGATATTCAAAAGGCTCAAGTCTCTGGTCAGGGTGATTCTTTGCACGAACACATCGCTAACTTGGCTGGTTCTCCAGCTATCAAAAAAGGTATTTTGCAAACTGTTAAGGTTGTTGACGAATTGGTTAAGGTTATGGGTAGACACAAGCCAGAGAATATTGTCATCGAGATGGCTAGAGAAAATCAAACCACTCAAAAGGGTCAAAAGAACTCCAGAGAGAGAATGAAAAGAATTGAGGAAGGTATCAAGGAGTTGGGTTCCCAAATTTTGAAGGAACACCCAGTCGAGAACACTCAATTGCAAAATGAAAAGTTGTACTTGTATTACTTGCAAAACGGTAGAGATATGTACGTTGACCAAGAATTGGACATTAACAGATTGTCTGACTACGATGTTGATCACATCGTTCCACAATCCTTTTTGAAGGATGACTCTATCGATAACAAGGTTTTGACTAGATCCGACAAGAATAGAGGTAAGTCTGATAACGTTCCATCTGAAGAAGTCGTCAAGAAGATGAAGAACTACTGGAGACAGTTGTTGAATGCTAAGTTGATTACTCAAAGAAAGTTCGATAACTTGACTAAGGCTGAGAGAGGTGGTTTGTCTGAGTTGGATAAGGCCGGTTTCATTAAGAGACAATTGGTCGAAACCAGACAAATCACCAAGCACGTTGCTCAAATCTTGGACTCTAGAATGAACACCAAGTACGACGAAAACGACAAATTGATTAGAGAGGTTAAGGTCATCACCTTGAAGTCCAAATTGGTTTCCGACTTTAGAAAGGATTTCCAATTCTACAAGGTCAGAGAAATCAATAACTACCACCATGCTCACGACGCCTACTTGAACGCTGTTGTCGGTACTGCTTTGATCAAGAAGTACCCAAAGTTGGAGTCTGAATTTGTCTACGGTGATTACAAAGTTTACGACGTTAGAAAGATGATTGCTAAGTCTGAGCAAGAAATCGGTAAGGCTACTGCTAAATACTTCTTCTACTCCAACATTATGAATTTCTTCAAGACCGAGATTACCTTGGCCAACGGTGAAATCAGAAAGAGACCATTGATTGAAACTAACGGTGAAACCGGTGAGATTGTCTGGGACAAGGGTAGAGATTTCGCTACTGTTAGAAAGGTCTTGTCTATGCCACAAGTCAACATTGTCAAGAAGACCGAAGTCCAGACTGGTGGTTTCTCCAAGGAATCTATTTTGCCAAAGAGAAACTCTGATAAGTTGATTGCCAGAAAGAAAGACTGGGATCCAAAGAAGTACGGTGGTTTCGACTCTCCAACCGTTGCTTACTCCGTTTTGGTTGTCGCTAAGGTTGAAAAGGGTAAGTCCAAGAAGTTGAAGTCCGTCAAGGAGTTGTTGGGTATTACCATCATGGAAAGATCTTCCTTCGAAAAGAACCCAATTGATTTCTTGGAGGCTAAGGGTTACAAGGAAGTCAAGAAGGACTTGATCATTAAGTTGCCAAAGTACTCTTTGTTCGAATTGGAGAACGGTAGAAAGAGAATGTTGGCTTCCGCTGGTGAATTGCAAAAGGGTAACGAATTGGCTTTGCCATCTAAGTACGTTAACTTCTTGTACTTGGCTTCCCACTACGAAAAGTTGAAGGGTTCTCCAGAAGATAACGAACAAAAGCAATTGTTCGTTGAACAACATAAGCACTACTTGGACGAAATCATCGAACAAATCTCTGAATTCTCTAAGAGAGTTATCTTGGCTGACGCTAACTTGGACAAGGTTTTGTCTGCTTACAACAAGCACAGAGACAAGCCAATCAGAGAGCAAGCTGAAAACATCATCCACTTGTTCACTTTGACCAACTTGGGTGCTCCAGCTGCTTTCAAGTACTTCGACACCACCATCGACAGAAAGAGATACACCTCTACCAAGGAAGTTTTGGACGCTACCTTGATCCACCAATCTATCACCGGTTTGTACGAAACCAGAATCGACTTGTCTCAATTGGGTGGTGAC

Protein Sequence (795 aa)

CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

pIW447-CRISPR XYL2 98906

2340..4725 (Fwd)

DNA Sequence (2385 bp)

Protein Sequence (795 aa)

pIW601-KmCRISPR 98907

2275..4660 (Fwd)

DNA Sequence (2385 bp)

Protein Sequence (795 aa)