| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| Cas9(C) | — | C-terminal portion of Streptococcus pyo… | 2275..4660 | Fwd | |
|
DNA Sequence
TGTTTCGACTCTGTCGAAATTTCTGGTGTCGAAGACAGATTCAATGCTTCTTTGGGTACTTACCACGATTTGTTGAAAATCATTAAGGATAAGGATTTCTTAGATAACGAGGAGAACGAAGATATTTTGGAGGATATTGTCTTGACTTTGACTTTGTTTGAGGATAGAGAAATGATCGAGGAAAGATTGAAGACTTACGCTCATTTGTTCGATGATAAGGTCATGAAGCAATTGAAGAGAAGAAGATACACTGGTTGGGGTAGATTGTCCAGAAAGTTGATCAACGGTATTAGAGATAAGCAATCCGGTAAGACCATTTTGGATTTCTTGAAGTCTGACGGTTTCGCTAATAGAAACTTTATGCAATTGATTCACGACGATTCTTTGACTTTTAAGGAAGATATTCAAAAGGCTCAAGTCTCTGGTCAGGGTGATTCTTTGCACGAACACATCGCTAACTTGGCTGGTTCTCCAGCTATCAAAAAAGGTATTTTGCAAACTGTTAAGGTTGTTGACGAATTGGTTAAGGTTATGGGTAGACACAAGCCAGAGAATATTGTCATCGAGATGGCTAGAGAAAATCAAACCACTCAAAAGGGTCAAAAGAACTCCAGAGAGAGAATGAAAAGAATTGAGGAAGGTATCAAGGAGTTGGGTTCCCAAATTTTGAAGGAACACCCAGTCGAGAACACTCAATTGCAAAATGAAAAGTTGTACTTGTATTACTTGCAAAACGGTAGAGATATGTACGTTGACCAAGAATTGGACATTAACAGATTGTCTGACTACGATGTTGATCACATCGTTCCACAATCCTTTTTGAAGGATGACTCTATCGATAACAAGGTTTTGACTAGATCCGACAAGAATAGAGGTAAGTCTGATAACGTTCCATCTGAAGAAGTCGTCAAGAAGATGAAGAACTACTGGAGACAGTTGTTGAATGCTAAGTTGATTACTCAAAGAAAGTTCGATAACTTGACTAAGGCTGAGAGAGGTGGTTTGTCTGAGTTGGATAAGGCCGGTTTCATTAAGAGACAATTGGTCGAAACCAGACAAATCACCAAGCACGTTGCTCAAATCTTGGACTCTAGAATGAACACCAAGTACGACGAAAACGACAAATTGATTAGAGAGGTTAAGGTCATCACCTTGAAGTCCAAATTGGTTTCCGACTTTAGAAAGGATTTCCAATTCTACAAGGTCAGAGAAATCAATAACTACCACCATGCTCACGACGCCTACTTGAACGCTGTTGTCGGTACTGCTTTGATCAAGAAGTACCCAAAGTTGGAGTCTGAATTTGTCTACGGTGATTACAAAGTTTACGACGTTAGAAAGATGATTGCTAAGTCTGAGCAAGAAATCGGTAAGGCTACTGCTAAATACTTCTTCTACTCCAACATTATGAATTTCTTCAAGACCGAGATTACCTTGGCCAACGGTGAAATCAGAAAGAGACCATTGATTGAAACTAACGGTGAAACCGGTGAGATTGTCTGGGACAAGGGTAGAGATTTCGCTACTGTTAGAAAGGTCTTGTCTATGCCACAAGTCAACATTGTCAAGAAGACCGAAGTCCAGACTGGTGGTTTCTCCAAGGAATCTATTTTGCCAAAGAGAAACTCTGATAAGTTGATTGCCAGAAAGAAAGACTGGGATCCAAAGAAGTACGGTGGTTTCGACTCTCCAACCGTTGCTTACTCCGTTTTGGTTGTCGCTAAGGTTGAAAAGGGTAAGTCCAAGAAGTTGAAGTCCGTCAAGGAGTTGTTGGGTATTACCATCATGGAAAGATCTTCCTTCGAAAAGAACCCAATTGATTTCTTGGAGGCTAAGGGTTACAAGGAAGTCAAGAAGGACTTGATCATTAAGTTGCCAAAGTACTCTTTGTTCGAATTGGAGAACGGTAGAAAGAGAATGTTGGCTTCCGCTGGTGAATTGCAAAAGGGTAACGAATTGGCTTTGCCATCTAAGTACGTTAACTTCTTGTACTTGGCTTCCCACTACGAAAAGTTGAAGGGTTCTCCAGAAGATAACGAACAAAAGCAATTGTTCGTTGAACAACATAAGCACTACTTGGACGAAATCATCGAACAAATCTCTGAATTCTCTAAGAGAGTTATCTTGGCTGACGCTAACTTGGACAAGGTTTTGTCTGCTTACAACAAGCACAGAGACAAGCCAATCAGAGAGCAAGCTGAAAACATCATCCACTTGTTCACTTTGACCAACTTGGGTGCTCCAGCTGCTTTCAAGTACTTCGACACCACCATCGACAGAAAGAGATACACCTCTACCAAGGAAGTTTTGGACGCTACCTTGATCCACCAATCTATCACCGGTTTGTACGAAACCAGAATCGACTTGTCTCAATTGGGTGGTGAC
Protein Sequence
CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 4672..4693 | Fwd | |
|
DNA Sequence
CCAAAGAAGAAGAGAAAGGTT
Protein Sequence
PKKKRKV
|
|||||
| AmpR | bla | beta-lactamase | 6885..7746 | Rev | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| URA3 | S. cerevisiae URA3 | orotidine-5'-phosphate decarboxylase, r… | 9647..10451 | Fwd | |
|
DNA Sequence
ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA
Protein Sequence
MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 91..110 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| TEF1 promoter | S. cerevisiae TEF1 | — | 126..525 | Fwd | |
|
DNA Sequence
CCTAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG
|
|||||
| T3 promoter | — | — | 5685..5704 | Rev | |
|
DNA Sequence
AATTAACCCTCACTAAAGG
|
|||||
| lac promoter | — | — | 5772..5803 | Rev | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| AmpR promoter | bla | — | 7746..7851 | Rev | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| URA3 promoter | S. cerevisiae URA3 | — | 9431..9647 | Fwd | |
|
DNA Sequence
TTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 5748..5765 | Fwd | |
|
DNA Sequence
TTGTTATCCGCTCACAA
|
|||||
| CAP binding site | — | — | 5817..5839 | Fwd | |
|
DNA Sequence
ATGAGTGAGCTAACTCACATTA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 6126..6715 | Rev | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| f1 ori | — | — | 10581..11037 | Rev | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| CYC1 terminator | S. cerevisiae CYC1 | — | 4708..4956 | Fwd | |
|
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC
|
|||||
| SUP4 terminator | — | — | 5641..5661 | Fwd | |
|
DNA Sequence
TTTTTTTGTTTTTTATGTCT
|
|||||