| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| Cas9(C) | — | C-terminal portion of Streptococcus pyo… | 2340..4725 | Fwd | |
|
DNA Sequence
TGTTTCGACTCTGTCGAAATTTCTGGTGTCGAAGACAGATTCAATGCTTCTTTGGGTACTTACCACGATTTGTTGAAAATCATTAAGGATAAGGATTTCTTAGATAACGAGGAGAACGAAGATATTTTGGAGGATATTGTCTTGACTTTGACTTTGTTTGAGGATAGAGAAATGATCGAGGAAAGATTGAAGACTTACGCTCATTTGTTCGATGATAAGGTCATGAAGCAATTGAAGAGAAGAAGATACACTGGTTGGGGTAGATTGTCCAGAAAGTTGATCAACGGTATTAGAGATAAGCAATCCGGTAAGACCATTTTGGATTTCTTGAAGTCTGACGGTTTCGCTAATAGAAACTTTATGCAATTGATTCACGACGATTCTTTGACTTTTAAGGAAGATATTCAAAAGGCTCAAGTCTCTGGTCAGGGTGATTCTTTGCACGAACACATCGCTAACTTGGCTGGTTCTCCAGCTATCAAAAAAGGTATTTTGCAAACTGTTAAGGTTGTTGACGAATTGGTTAAGGTTATGGGTAGACACAAGCCAGAGAATATTGTCATCGAGATGGCTAGAGAAAATCAAACCACTCAAAAGGGTCAAAAGAACTCCAGAGAGAGAATGAAAAGAATTGAGGAAGGTATCAAGGAGTTGGGTTCCCAAATTTTGAAGGAACACCCAGTCGAGAACACTCAATTGCAAAATGAAAAGTTGTACTTGTATTACTTGCAAAACGGTAGAGATATGTACGTTGACCAAGAATTGGACATTAACAGATTGTCTGACTACGATGTTGATCACATCGTTCCACAATCCTTTTTGAAGGATGACTCTATCGATAACAAGGTTTTGACTAGATCCGACAAGAATAGAGGTAAGTCTGATAACGTTCCATCTGAAGAAGTCGTCAAGAAGATGAAGAACTACTGGAGACAGTTGTTGAATGCTAAGTTGATTACTCAAAGAAAGTTCGATAACTTGACTAAGGCTGAGAGAGGTGGTTTGTCTGAGTTGGATAAGGCCGGTTTCATTAAGAGACAATTGGTCGAAACCAGACAAATCACCAAGCACGTTGCTCAAATCTTGGACTCTAGAATGAACACCAAGTACGACGAAAACGACAAATTGATTAGAGAGGTTAAGGTCATCACCTTGAAGTCCAAATTGGTTTCCGACTTTAGAAAGGATTTCCAATTCTACAAGGTCAGAGAAATCAATAACTACCACCATGCTCACGACGCCTACTTGAACGCTGTTGTCGGTACTGCTTTGATCAAGAAGTACCCAAAGTTGGAGTCTGAATTTGTCTACGGTGATTACAAAGTTTACGACGTTAGAAAGATGATTGCTAAGTCTGAGCAAGAAATCGGTAAGGCTACTGCTAAATACTTCTTCTACTCCAACATTATGAATTTCTTCAAGACCGAGATTACCTTGGCCAACGGTGAAATCAGAAAGAGACCATTGATTGAAACTAACGGTGAAACCGGTGAGATTGTCTGGGACAAGGGTAGAGATTTCGCTACTGTTAGAAAGGTCTTGTCTATGCCACAAGTCAACATTGTCAAGAAGACCGAAGTCCAGACTGGTGGTTTCTCCAAGGAATCTATTTTGCCAAAGAGAAACTCTGATAAGTTGATTGCCAGAAAGAAAGACTGGGATCCAAAGAAGTACGGTGGTTTCGACTCTCCAACCGTTGCTTACTCCGTTTTGGTTGTCGCTAAGGTTGAAAAGGGTAAGTCCAAGAAGTTGAAGTCCGTCAAGGAGTTGTTGGGTATTACCATCATGGAAAGATCTTCCTTCGAAAAGAACCCAATTGATTTCTTGGAGGCTAAGGGTTACAAGGAAGTCAAGAAGGACTTGATCATTAAGTTGCCAAAGTACTCTTTGTTCGAATTGGAGAACGGTAGAAAGAGAATGTTGGCTTCCGCTGGTGAATTGCAAAAGGGTAACGAATTGGCTTTGCCATCTAAGTACGTTAACTTCTTGTACTTGGCTTCCCACTACGAAAAGTTGAAGGGTTCTCCAGAAGATAACGAACAAAAGCAATTGTTCGTTGAACAACATAAGCACTACTTGGACGAAATCATCGAACAAATCTCTGAATTCTCTAAGAGAGTTATCTTGGCTGACGCTAACTTGGACAAGGTTTTGTCTGCTTACAACAAGCACAGAGACAAGCCAATCAGAGAGCAAGCTGAAAACATCATCCACTTGTTCACTTTGACCAACTTGGGTGCTCCAGCTGCTTTCAAGTACTTCGACACCACCATCGACAGAAAGAGATACACCTCTACCAAGGAAGTTTTGGACGCTACCTTGATCCACCAATCTATCACCGGTTTGTACGAAACCAGAATCGACTTGTCTCAATTGGGTGGTGAC
Protein Sequence
CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 4737..4758 | Fwd | |
|
DNA Sequence
CCAAAGAAGAAGAGAAAGGTT
Protein Sequence
PKKKRKV
|
|||||
| AmpR | bla | beta-lactamase | 6970..7831 | Rev | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| URA3 | S. cerevisiae URA3 | orotidine-5'-phosphate decarboxylase, r… | 9732..10536 | Fwd | |
|
DNA Sequence
ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA
Protein Sequence
MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 156..175 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| TEF1 promoter | S. cerevisiae TEF1 | — | 191..590 | Fwd | |
|
DNA Sequence
CCTAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG
|
|||||
| T3 promoter | — | — | 5770..5789 | Rev | |
|
DNA Sequence
AATTAACCCTCACTAAAGG
|
|||||
| lac promoter | — | — | 5857..5888 | Rev | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| AmpR promoter | bla | — | 7831..7936 | Rev | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| URA3 promoter | S. cerevisiae URA3 | — | 9516..9732 | Fwd | |
|
DNA Sequence
TTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 5833..5850 | Fwd | |
|
DNA Sequence
TTGTTATCCGCTCACAA
|
|||||
| CAP binding site | — | — | 5902..5924 | Fwd | |
|
DNA Sequence
ATGAGTGAGCTAACTCACATTA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 6211..6800 | Rev | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| f1 ori | — | — | 10666..11122 | Rev | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| CYC1 terminator | S. cerevisiae CYC1 | — | 4773..5021 | Fwd | |
|
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC
|
|||||
| SUP4 terminator | — | — | 5726..5746 | Fwd | |
|
DNA Sequence
TTTTTTTGTTTTTTATGTCT
|
|||||