OeNL_pRSETb

89529 Synthetic Bacterial Expression

Plasmid Information

Purpose
Orange color variant of bright luminescent protein for bacterial expression
Depositor
Takeharu Nagai
Article
Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10
Insert
OeNL
Tags
His X 6
Promoter
T7
Available Since
April 19, 2017
Availability
Available

Sequence Information

Length
4,123 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 6
Label Gene Product Position Strand
mKO2 monomeric Kusabira-Orange 2 fluorescent… 0..4123 Fwd
DNA Sequence
GTGAGCGTGATCAAGCCCGAGATGAAGATGAGGTACTACATGGACGGCTCCGTCAATGGGCATGAGTTCACAATCGAGGGTGAGGGCACAGGCAGACCTTACGAGGGACATCAGGAGATGACACTGCGCGTCACAATGGCCGAGGGCGGGCCAATGCCTTTCGCCTTCGACCTGGTGTCCCACGTGTTCTGTTACGGCCACAGAGTTTTTACTAAATATCCAGAAGAGATCCCAGACTATTTCAAGCAGGCCTTTCCTGAGGGCCTGTCCTGGGAGAGGTCCCTGGAGTTCGAGGACGGCGGCTCCGCCTCCGTGAGCGCCCACATCAGCCTGAGGGGCAACACCTTCTACCACAAGTCCAAGTTCACCGGCGTGAACTTCCCCGCCGACGGCCCCATCATGCAGAACCAGAGCGTGGACTGGGAGCCCTCCACCGAGAAGATCACCGCCAGCGACGGCGTGCTGAAGGGCGACGTGACCATGTACCTGAAGCTGGAGGGCGGCGGCAACCACAAGTGCCAGATGAAGACCACCTACAAGGCCGCCAAGGAGATCCTGGAGATGCCCGGCGACCACTACATCGGCCACAGGCTGGTGAGGAAGACCGAGGGCAACATCACCGAGCAGGTGGAGGACGCCGTGGCCCACTCC
Protein Sequence
VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS
hmKO humanized monomeric Kusabira-Orange flu… 0..4123 Fwd
DNA Sequence
GTGAGCGTGATCAAGCCCGAGATGAAGATGAGGTACTACATGGACGGCTCCGTCAATGGGCATGAGTTCACAATCGAGGGTGAGGGCACAGGCAGACCTTACGAGGGACATCAGGAGATGACACTGCGCGTCACAATGGCCGAGGGCGGGCCAATGCCTTTCGCCTTCGACCTGGTGTCCCACGTGTTCTGTTACGGCCACAGAGTTTTTACTAAATATCCAGAAGAGATCCCAGACTATTTCAAGCAGGCCTTTCCTGAGGGCCTGTCCTGGGAGAGGTCCCTGGAGTTCGAGGACGGCGGCTCCGCCTCCGTGAGCGCCCACATCAGCCTGAGGGGCAACACCTTCTACCACAAGTCCAAGTTCACCGGCGTGAACTTCCCCGCCGACGGCCCCATCATGCAGAACCAGAGCGTGGACTGGGAGCCCTCCACCGAGAAGATCACCGCCAGCGACGGCGTGCTGAAGGGCGACGTGACCATGTACCTGAAGCTGGAGGGCGGCGGCAACCACAAGTGCCAGATGAAGACCACCTACAAGGCCGCCAAGGAGATCCTGGAGATGCCCGGCGACCACTACATCGGCCACAGGCTGGTGAGGAAGACCGAGGGCAACATCACCGAGCAGGTGGAGGACGCCGTGGCCCACTCC
Protein Sequence
VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS
AmpR bla beta-lactamase 1798..2659 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
6xHis 6xHis affinity tag 3765..3783 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 3786..3819 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 3822..3846 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
promoter 2
Label Gene Product Position Strand
AmpR promoter bla 1693..1798 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
T7 promoter 3673..3692 Fwd
DNA Sequence
TAATACGACTCACTATAGG
rep_origin 2
Label Gene Product Position Strand
f1 ori 1212..1668 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
ori 2829..3418 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator 1068..1116 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG