| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 131..149 | Fwd | |
|
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 1289..1310 | Fwd | |
|
DNA Sequence
CCAAAAAAGAAGAGAAAGGTA
Protein Sequence
PKKKRKV
|
|||||
| AsCpf1 | — | CRISPR-associated protein from Acidamin… | 1316..5237 | Fwd | |
|
DNA Sequence
ATGACACAGTTTGAAGGCTTCACCAATCTCTACCAGGTCAGCAAGACGCTACGTTTTGAGCTTATCCCGCAGGGAAAAACCCTGAAACACATTCAGGAACAGGGGTTCATAGAGGAAGATAAGGCGCGTAACGACCATTATAAAGAACTGAAGCCTATAATCGACCGTATTTATAAAACGTACGCGGATCAGTGCCTGCAGCTGGTTCAGCTGGATTGGGAGAATCTGTCCGCGGCTATTGATAGCTATCGCAAAGAGAAGACCGAGGAAACCCGTAACGCACTGATTGAAGAGCAGGCGACCTATCGGAATGCGATCCATGATTACTTCATCGGCCGCACCGACAACCTGACCGATGCAATTAACAAACGTCACGCAGAGATTTACAAAGGTCTGTTTAAAGCAGAGTTATTCAATGGCAAGGTTCTGAAACAGCTGGGTACGGTCACCACCACCGAACACGAAAACGCACTGCTGAGGAGCTTTGATAAATTTACCACATATTTCAGCGGTTTCTATGAAAATCGTAAGAATGTATTTAGCGCCGAAGATATTTCCACCGCAATTCCTCATCGTATTGTGCAGGATAATTTTCCGAAGTTTAAAGAAAATTGTCATATTTTTACCCGTCTGATCACCGCGGTACCGAGCCTGCGAGAGCATTTTGAAAACGTTAAGAAAGCCATTGGAATTTTTGTCAGTACCAGCATTGAAGAAGTGTTTTCGTTCCCGTTCTATAACCAACTGCTGACCCAGACCCAGATTGATCTGTACAATCAGCTGCTGGGGGGCATAAGCCGCGAGGCAGGTACCGAAAAGATAAAGGGACTCAATGAGGTGCTGAATCTGGCAATTCAGAAGAATGATGAAACGGCTCATATCATTGCTAGCCTGCCGCATCGTTTCATTCCCCTGTTTAAGCAAATCCTGAGCGATCGCAATACACTGAGCTTTATCCTCGAAGAGTTTAAATCGGACGAAGAAGTTATCCAGAGCTTTTGCAAATACAAAACCCTGCTGCGGAACGAAAATGTGCTGGAGACCGCTGAAGCACTGTTTAATGAACTGAACTCGATCGACCTCACCCATATTTTTATATCCCACAAAAAACTGGAAACCATAAGCAGCGCTCTGTGTGACCATTGGGATACCCTGCGCAACGCCCTGTATGAACGGCGTATCAGCGAGCTGACCGGGAAAATCACCAAATCCGCAAAGGAAAAAGTTCAGCGTAGTCTGAAACACGAGGACATCAACCTGCAAGAAATTATTAGCGCAGCAGGTAAAGAGCTGAGCGAAGCATTCAAACAGAAAACCAGCGAAATCCTGAGCCATGCCCATGCTGCACTGGATCAGCCGCTGCCGACCACCCTGAAAAAACAGGAGGAAAAGGAGATTCTGAAAAGCCAACTGGACAGCCTGCTGGGCCTGTATCACCTGCTGGACTGGTTTGCAGTCGATGAGAGCAACGAGGTTGATCCTGAGTTCTCCGCTCGTCTGACCGGAATCAAGCTGGAGATGGAACCGAGTCTGTCGTTTTACAATAAAGCGCGTAATTACGCGACCAAGAAACCGTATAGCGTGGAAAAATTCAAACTGAACTTTCAGATGCCGACCCTTGCAAGCGGATGGGACGTTAACAAAGAAAAAAACAATGGGGCAATTCTGTTTGTGAAAAATGGCCTCTATTATCTGGGTATCATGCCGAAACAGAAAGGGCGCTACAAAGCCCTGTCATTTGAGCCGACCGAGAAAACCTCAGAGGGTTTCGACAAGATGTACTACGATTATTTCCCGGATGCGGCAAAAATGATACCCAAATGTAGCACCCAACTGAAGGCAGTTACAGCCCACTTTCAGACCCATACCACCCCGATCCTGCTGTCGAACAATTTTATAGAGCCGCTGGAAATTACCAAAGAGATTTATGATCTGAATAATCCGGAAAAGGAGCCCAAGAAATTTCAGACGGCGTATGCAAAAAAGACCGGGGATCAGAAAGGTTATCGTGAAGCGCTGTGCAAATGGATTGACTTTACCCGTGACTTTCTGTCAAAATATACCAAAACGACGAGCATTGATCTGAGCAGCCTACGTCCGAGCAGCCAATATAAGGATCTGGGCGAATATTACGCCGAACTGAATCCGCTGCTCTACCATATTTCCTTCCAACGAATCGCTGAAAAAGAAATAATGGACGCCGTTGAAACCGGCAAACTGTATCTGTTTCAAATCTACAACAAAGATTTCGCCAAAGGCCATCACGGTAAGCCGAACCTGCATACCCTGTATTGGACCGGTCTGTTTAGCCCGGAGAATCTGGCCAAAACCAGCATCAAGCTGAACGGACAGGCAGAACTGTTTTACCGCCCCAAAAGCCGTATGAAAAGGATGGCACACCGCCTGGGCGAAAAAATGCTGAATAAGAAACTCAAAGATCAGAAAACGCCGATACCGGATACCCTTTATCAGGAGCTGTATGATTATGTTAACCACCGGCTGAGCCATGACCTGAGCGACGAAGCGCGTGCACTGCTGCCGAACGTGATTACCAAGGAAGTCTCGCATGAAATTATTAAAGATCGGCGCTTCACCAGTGATAAATTTTTCTTCCATGTACCGATCACCCTGAATTATCAAGCCGCAAATAGCCCTTCCAAATTTAATCAACGCGTGAATGCGTACCTGAAAGAGCATCCGGAGACCCCAATTATTGGCATAGACCGAGGAGAACGCAATCTCATTTATATCACCGTCATTGATAGCACCGGTAAGATCCTGGAACAGCGTAGCCTGAATACCATTCAGCAGTTTGACTACCAGAAAAAGCTGGACAACAGAGAAAAGGAACGTGTAGCCGCCCGGCAGGCTTGGAGTGTGGTGGGTACTATCAAGGATCTGAAGCAGGGGTATCTCTCCCAAGTTATCCATGAAATTGTCGATCTAATGATTCACTATCAAGCAGTAGTGGTACTGGAAAATCTGAATTTCGGTTTCAAAAGCAAACGTACAGGGATCGCTGAAAAAGCCGTTTATCAGCAGTTCGAGAAAATGCTGATAGACAAGCTGAATTGCCTGGTTCTGAAAGATTATCCGGCAGAGAAGGTGGGCGGTGTGCTGAACCCGTACCAGCTGACTGATCAATTTACGAGCTTTGCAAAAATGGGAACGCAGAGCGGTTTCCTGTTCTATGTTCCGGCGCCATATACCAGCAAGATAGACCCGCTGACAGGTTTCGTAGATCCGTTTGTCTGGAAAACCATTAAAAATCATGAAAGTCGCAAACATTTTCTGGAGGGCTTTGATTTTCTGCACTATGACGTGAAAACCGGCGACTTCATTCTGCATTTTAAAATGAACCGTAATCTGTCCTTTCAGCGCGGCCTGCCTGGCTTTATGCCGGCGTGGGACATTGTTTTTGAAAAGAATGAGACACAGTTTGATGCCAAAGGTACCCCCTTTATTGCGGGGAAACGCATTGTGCCCGTTATAGAAAATCACCGCTTCACCGGACGGTATAGGGACTTGTACCCGGCAAATGAATTGATAGCGCTGCTGGAGGAGAAAGGTATTGTCTTTCGGGATGGATCAAACATCCTGCCGAAGCTGCTGGAGAACGATGACAGCCACGCAATAGACACCATGGTAGCGCTGATCCGAAGCGTGCTGCAGATGCGTAACAGTAATGCGGCTACGGGGGAAGACTACATTAATAGCCCGGTCCGTGATCTGAACGGCGTTTGTTTCGATAGCAGATTTCAAAATCCGGAGTGGCCGATGGATGCCGATGCCAATGGAGCTTACCATATCGCTCTCAAAGGTCAGCTCCTACTGAACCATTTGAAAGAATCAAAAGATCTGAAACTGCAGAACGGCATCTCGAATCAGGACTGGCTGGCCTACATTCAAGAACTGAGAAAC
Protein Sequence
MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 5237..5258 | Fwd | |
|
DNA Sequence
CCAAAGAAAAAGAGAAAAGTA
Protein Sequence
PKKKRKV
|
|||||
| HA | — | HA (human influenza hemagglutinin) epit… | 5264..5291 | Fwd | |
|
DNA Sequence
TACCCCTACGACGTGCCCGACTACGCC
Protein Sequence
YPYDVPDYA
|
|||||
| AmpR | bla | beta-lactamase | 6012..6873 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTCCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| rop | rop | Rop protein, which maintains plasmids a… | 8554..8746 | Rev | |
|
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTGCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
|
|||||
| lacI | lacI | lac repressor | 9319..10402 | Fwd | |
|
DNA Sequence
GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA
Protein Sequence
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| tac promoter | — | — | 0..29 | Fwd | |
|
DNA Sequence
TTGACAATTAATCATCGGCTCGTATAATG
|
|||||
| AmpR promoter | bla | — | 5920..6012 | Fwd | |
|
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| lacIq promoter | lacI (mutant) | — | 9241..9319 | Fwd | |
|
DNA Sequence
GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 36..53 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| attB1 | mutant version of attB | — | 1250..1275 | Fwd | |
|
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
|
|||||
| attB2 | mutant version of attB | — | 5295..5320 | Rev | |
|
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| M13 ori | — | — | 6914..7428 | Rev | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCGGGGT
|
|||||
| ori | — | — | 7538..8127 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| rrnB T1 terminator | Escherichia coli rrnB | — | 5696..5783 | Fwd | |
|
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT
|
|||||
| rrnB T2 terminator | — | — | 5874..5902 | Fwd | |
|
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
|
|||||