pET28a-Hook3 Q147A, M151A, I154A

74616 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses a triple mutant truncation of Hook3 in bacteria for purification
Depositor
Ron Vale
Article
Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d
Insert
human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
Tags
6x His, Strep II, and superfolder GFP
Mutation
Q147A, M151A, I154A
Promoter
T7
Available Since
Aug. 5, 2016
Availability
Available

Sequence Information

Length
863 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 166..184 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 193..211 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 214..247 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Twin-Strep-tag — two Strep-Tag(R)II moieties connected b… 262..346 Fwd
DNA Sequence
TGGAGTCATCCACAATTTGAGAAGGGCGGCGGCAGTGGCGGCGGCAGTGGCGGCAGTGCCTGGAGCCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEKGGGSGGGSGGSAWSHPQFEK
promoter 1
Label Gene Product Position Strand
T7 promoter — — 68..87 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 87..112 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC