pET28a-Hook3 aa 1-239

74609 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification
Depositor
Ron Vale
Article
Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d
Insert
human Hook3 amino acids 1-239 (HOOK3 Human)
Tags
6x His, Strep II, and superfolder GFP
Available Since
Aug. 5, 2016
Availability
Available

Sequence Information

Length
1,066 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 167..185 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 194..212 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 215..248 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Twin-Strep-tag — two Strep-Tag(R)II moieties connected b… 263..347 Fwd
DNA Sequence
TGGAGTCATCCACAATTTGAGAAGGGCGGCGGCAGTGGCGGCGGCAGTGGCGGCAGTGCCTGGAGCCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEKGGGSGGGSGGSAWSHPQFEK
superfolder GFP — GFP variant that folds robustly even wh… 353..1064 Fwd
DNA Sequence
ATGAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCCGTGGAGAGGGTGAAGGTGATGCTACAAACGGAAAACTCACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCGTGGCCAACACTTGTCACTACTCTGACCTATGGTGTTCAATGCTTTTCCCGTTATCCGGATCACATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACTATATCTTTCAAAGATGACGGGACCTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAGTTAAAGGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAACTCGAATACAACTTTAACTCACACAATGTATACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTCGCCACAACGTTGAAGATGGTTCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCGACACAATCTGTCCTTTCGAAAGATCCCAACGAAAAGCGTGACCACATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTAC
Protein Sequence
MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELY
promoter 1
Label Gene Product Position Strand
T7 promoter — — 69..88 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 88..113 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC