Kohinoor/pRSETb

67770 Synthetic Bacterial Expression

Plasmid Information

Purpose
Kohinoor for bacterial expression
Depositor
Takeharu Nagai
Article
Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:
Insert
Kohinoor
Tags
6xHis
Promoter
T7
Available Since
Sept. 10, 2015
Availability
Available

Sequence Information

Length
870 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 51..69 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 72..105 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 108..132 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
Kohinoor fast-switching photoswitchable fluoresc… 141..816 Fwd
DNA Sequence
ATGAGTGTGATTAAACCAGACATGAAGATCAAGCTGCGTATGGAAGGCGCTGTAAATGGACACCCGTTCGCGATTGAAGGAGTTGGCCTTGGGAAGCCTTTCGAGGGAAAACAGAGTATGGACCTTAAAGTCAAAGAAGGCGGACCTCTGCCTTTCGCCTATGACATCTTGACAATGGCCTTCTGTTACGGCAACAGGGTATTCGCCAAATACCCAGAAAATATAGTAGATTATTTCAAGCAGTCGTTTCCTGAGGGCTACTCTTGGGAACGAAGCATGATTTACGAAGACGGGGGCATTTGTATCGCGACAAACGACATAACCCTGGATGGTGACTGTTATATCTATGAAATTCGATTTGATGGTGTGAACTTTCCTGCCAATGGTCCAGTTATGCAGAAGAGGACTGTGAAATGGGAGCCATCCACTGAGAAATTGTATGTGCGTGATGGAGTGCTGAAGAGCGATGGCAACTACGCTCTGTCGCTTGAAGGAGGTGGCCACTACCGATGTGACTCCAAAACTACTTATAAAGCTAAGAAGGTTGTCCAGTTGCCAGACTATCACGATGTGGTCCACCACATTGAGATTAAAAGCCACGACAGAGATTACAGTAATGTTAATCTGCATGAGCATGCCGAAGCGCATTCTGGGCTGCCGAGGCAGGCCAAGTAA
Protein Sequence
MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVKEGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIATNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSLEGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQAK