pRSET-triple mutation-pa-GCaMP6f

67570 Synthetic Bacterial Expression

Plasmid Information

Purpose
bacterial expression of a photoactivatable fluorescent calcium reporter
Depositor
Ehud Isacoff
Article
Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
Insert
pa-GCaMP6f
Tags
HIS-tag
Mutation
V115H, L64F, T65S
Promoter
T7
Available Since
Aug. 6, 2015
Availability
Available

Sequence Information

Length
786 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 39..57 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 60..93 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 96..120 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
calmodulin-binding peptide calmodulin-binding peptide from avian s… 147..204 Fwd
DNA Sequence
CGTCGTAAGTGGAATAAGACAGGTCACGCAGTCAGAGCTATAGGTCGGCTGAGCTCA
Protein Sequence
RRKWNKTGHAVRAIGRLSS