pRSET-spa-GCaMP6f

67563 Synthetic Bacterial Expression

Plasmid Information

Purpose
bacterial expression of a photoactivatable fluorescent calcium reporter
Depositor
Ehud Isacoff
Article
Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
Insert
spa-GCaMP6f
Tags
HIS-tag
Mutation
K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
Available Since
July 29, 2015
Availability
Available

Sequence Information

Length
826 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 30..48 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 51..84 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 87..111 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
calmodulin-binding peptide calmodulin-binding peptide from avian s… 138..195 Fwd
DNA Sequence
CGTCGTAAGTGGAATAAGACAGGTCACGCAGTCAGAGCTATAGGTCGGCTGAGCTCA
Protein Sequence
RRKWNKTGHAVRAIGRLSS