Fast-GCaMP6f-RS09

67160 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
Depositor
Samuel Wang
Article
Badura et al Neurophotonics. 2014 Oct;1(2):025008.
Insert
Fast-GCaMP6f-RS09
Tags
6xHis, T7 tag, and Xpress tag
Mutation
L414T within GCaMP6f
Promoter
T7
Available Since
July 7, 2015
Availability
Available

Sequence Information

Length
1,058 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 146..164 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 167..200 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 203..227 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
calmodulin-binding peptide — calmodulin-binding peptide from avian s… 254..311 Fwd
DNA Sequence
CGTCGTAAGTGGAATAAGACAGGTCACGCAGTCAGAGCTATAGGTCGGCTGAGCTCA
Protein Sequence
RRKWNKTGHAVRAIGRLSS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 57..76 Fwd
DNA Sequence
TAATACGACTCACTATAGG