pet28a_RBPMS_1_144

65739 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
plasmid for bacterial overexpression of RBPMS
Depositor
Thomas Tuschl
Article
Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12
Insert
RBPMS (RBPMS Human)
Tags
His Tag
Mutation
deletion construct encoding amino acids 1-144
Promoter
T7
Available Since
July 30, 2015
Availability
Available

Sequence Information

Length
737 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 41..59 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 68..86 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 89..122 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
6xHis — 6xHis affinity tag 602..620 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 686..734 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG