pet23a_RBPMS_1_120

65730 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
plasmid for bacterial overexpression of RBPMS
Depositor
Thomas Tuschl
Article
Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12
Insert
RBPMS (RBPMS Human)
Tags
His Tag
Mutation
deletion construct encoding amino acids 1-120
Promoter
T7
Available Since
June 18, 2015
Availability
Available

Sequence Information

Length
831 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 18..36 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 441..474 Rev
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 535..554 Rev
DNA Sequence
TAATACGACTCACTATAGG