pYTK058

65165 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes Venus as a Type 4a part to be used in the Dueber YTK system
Depositor
John Dueber
Article
Lee et al ACS Synth Biol. 2015 Apr 14.
Insert
Venus
Available Since
June 16, 2015
Availability
Available

Sequence Information

Length
1,031 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
yeGFP — yeast-enhanced green fluorescent protein 134..848 Fwd
DNA Sequence
TCTAAAGGTGAAGAATTATTCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTCTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTGATTTGTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTAGGTTATGGTTTGCAATGTTTTGCTAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCATGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGACCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTATTGATTTTAAAGAAGGTGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCACAATGTTTACATCACTGCTGACAAACAAAAGAATGGTATCAAAGCTAACTTCAAAATTAGACACAACATTGAAGATGGTGGTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAATTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCTATCAATCTGCCTTATCCAAAGATCCAAACGAAAAGAGAGATCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGGTATTACCCATGGTATGGATGAATTGTACAAATAA
Protein Sequence
SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 871..906 Rev
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT