pAc-­His-­zfTpo

64309 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Express soluble His-tagged zebrafish thrombopoietin in insect cells
Depositor
Petr Bartunek
Article
Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10
Insert
Thrombopoietin (thpo Zebrafish)
Tags
(His)6
Mutation
Thrombopoietin starts at AA37 (AA1-36 encode the …
Available Since
Sept. 18, 2015
Availability
Available

Sequence Information

Length
183 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 88..106 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
sig_peptide 1
Label Gene Product Position Strand
GP64 signal sequence Autographa californica gp64 signal sequence from the baculovirus en… 4..64 Fwd
DNA Sequence
ATGGTAAGCGCTATTGTTTTATATGTGCTTTTGGCGGCGGCGGCGCATTCTGCCTTTGCG