Human PNP his-tagged

64076 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human Purine Nucleoside Phosphorylase
Depositor
Vern Schramm
Article
Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8
Insert
Purine Nucleoside Phosphorylase (PNP Human)
Tags
Histidine tag
Available Since
April 29, 2015
Availability
Available

Sequence Information

Length
882 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 28..46 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 49..82 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 85..109 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK