pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

61941 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli
Depositor
Chris Hill
Article
VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1
Tags
6x HIS
Mutation
Construct ends at residue 117, stop codon introdu…
Promoter
T7
Available Since
Oct. 9, 2015
Availability
Available

Sequence Information

Length
884 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
S-Tag — affinity and epitope tag derived from p… 1..46 Rev
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 488..507 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 463..488 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC