pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

61940 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli
Depositor
Chris Hill
Article
VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1
Tags
6x HIS
Mutation
Construct ends at residue 156, residues 97-100 (N…
Promoter
T7
Available Since
Oct. 9, 2015
Availability
Available

Sequence Information

Length
837 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
S-Tag — affinity and epitope tag derived from p… 4..49 Rev
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 608..627 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 583..608 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC