pet42c(+)-GST-USP5

61750 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
express N-terminally GST tagged human USP5
Depositor
Yihong Ye
Article
Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife
Insert
USP5 (USP5 Human)
Tags
GST
Promoter
T7
Available Since
Feb. 5, 2015
Availability
Available

Sequence Information

Length
852 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 15..33 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 42..60 Fwd
DNA Sequence
CTGGTGCCACGCGGTAGT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 75..120 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
Factor Xa site Factor Xa recognition and cleavage site 150..162 Fwd
DNA Sequence
ATTGAGGGACGC
Protein Sequence
IEGR