pCRISPomyces-2

61737 Synthetic Bacterial Expression

Plasmid Information

Purpose
Streptomyces expression of codon-optimized Cas9 and custom gRNA
Depositor
Huimin Zhao
Article
Cobb et al ACS Synth Biol. 2014 Dec 8.
Mutation
BbsI-flanked lacZ cassette inserted in place of s…
Promoter
gapdhp(EL) and rpsL(XC)-BbsI
Available Since
Jan. 27, 2015
Availability
Available

Sequence Information

Length
10,994 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
pSG5 Rep rep replication initiator protein from the … 152..1595 Rev
DNA Sequence
ATGTGGAAGCCGGGCGAGGCGACGTGGGGCAACACGTGCCGCTGCAACAACGTCCACACCTGCCCGTGGTGCATGAGCCGCATCCTGGCCGTACGGGGCTCGAACGTGCAGCTCGCCGCCGACGGTCTCGCGGACGCCGGGTACGGGCTGCACCTTGGCACGAACACGTTGCGCCACTTCGAGCGCATGGCGTTCGGCACCGTCCGCAAGGGAATGCGGCACGGCCTGGTCGCGGTCTTGCACGACGGCTGGAAGGGCGCGTACGGCTCGTCCGGCCGCCGCTGGCGCACGATGCGCGACGACTTCGGGATCATCGGCTACGAGCGGGCCTTCGAGGACACCTTCGGCTGGGGCTCCGGCTGGCACCTGCACTGGCACACGCTCTGGGTCACCCGCGAGGTGCTCGGCCCGGACGCCCAGGCCGCGTTCCGCGACGCACTCGCCGGAGCCTGGGCCGCCGGGGTCGAATCCGCGGGCGGCTACACCGTCAGCGAGACGTGCGACCGGCCCGGTTGCTCCTGTGAGGGCAAGGGCCACGGCACCGACGTGCGCCCACTCAACGGCGCGGACGCCGCTGACGGCGATGCGGGCAAGCAGGCCCGCTACCTCTACAAGGACGGCGACAAGACCAAGGGCGGCGTCGCCAAGATCGGCCTGGAGCTGGCCGGACAGAACTTCAAGGCCGGTCGCGGCGACGACCGCATGGGACCGCTCGACCTGGGCGACGCGGCGGCCGCCGAGCTGCAGCGGCTTCGTCGGCCCGGCCCGTTCGTCGAGAAGTACCGCGAGCGCGAGTTCGGCGTGTTCCAGGTCCGCAAGCACTACCGGTCGCAGAACCTGAACCGGCTGATCAAGGAACTCGGCATCCAGCAGGACGTCCGCACCGAGGAGGAGATCACCGACGACACCGAGGGCCTGGTCGCCATCGCGGTCATCCCGGCCTACATCTGGTACCGATACATCGCCCGCGTCGCCGGACGTCGCCTCGACCTCATCAAGGTCGCCGAGACGTACGGCCTACCGGGCGTCCGGCGGCTCGTCGAGTCCTGGGGCCTGGTGTGGGGCAAGGACGTCCTGGACCCGCCCGCCCCGGAAGCGCCGGCCGCGCCGGGCGACCTTGACGCTGACCAGATGAGGTTCGAGGTCATGTCCGAGGAGGAAGCTGCTTTCCGCGAGGCGCGCCGGAAGGCCAACGAGGCCCGCACGGAGGAGCTGGCCGCTTCGCTCGACCGAGTGCGTCAGCCGAAGAAGGAGGCGATCCGCCCGACGATCAGCCTTCGGAAGCGCCTGAAGCCGAAGCCGGTCACCGTCGACGTCAAGACTCCCCCGCCCGGCGCCGCGTCGCCCGTGTGCCGCCGCTGCAAGGGCAAGCTCGCCCCGGTACTCCAGCCCTGGGGCCAGCACCCCGGAGACTGCCTCCGCGTCGACACAGCGGTCGCGTAG
Protein Sequence
MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLADAGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGIIGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTVSETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELAGQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLNRLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGLPGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANEARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKLAPVLQPWGQHPGDCLRVDTAVA
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 3394..7501 Fwd
DNA Sequence
ATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGACTGA
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 3394..7498 Fwd
DNA Sequence
ATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
lacZ-alpha lacZ fragment LacZ-alpha fragment of beta-galactosida… 7993..8290 Rev
DNA Sequence
ATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCTGA
Protein Sequence
MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG
ApmR aac(3)IV aminoglycoside 3-N-acetyltransferase ty… 9407..10208 Fwd
DNA Sequence
ATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCTTCTCAACCTTGGGGTTACCCCCGGCGGTGTGCTGCTGGTCCACAGCTCCTTCCGTAGCGTCCGGCCCCTCGAAGATGGGCCACTTGGACTGATCGAGGCCCTGCGTGCTGCGCTGGGTCCGGGAGGGACGCTCGTCATGCCCTCGTGGTCAGGTCTGGACGACGAGCCGTTCGATCCTGCCACGTCGCCCGTTACACCGGACCTTGGAGTTGTCTCTGACACATTCTGGCGCCTGCCAAATGTAAAGCGCAGCGCCCATCCATTTGCCTTTGCGGCAGCGGGGCCACAGGCAGAGCAGATCATCTCTGATCCATTGCCCCTGCCACCTCACTCGCCTGCAAGCCCGGTCGCCCGTGTCCATGAACTCGATGGGCAGGTACTTCTCCTCGGCGTGGGACACGATGCCAACACGACGCTGCATCTTGCCGAGTTGATGGCAAAGGTTCCCTATGGGGTGCCGAGACACTGCACCATTCTTCAGGATGGCAAGTTGGTACGCGTCGATTATCTCGAGAATGACCACTGCTGTGAGCGCTTTGCCTTGGCGGACAGGTGGCTCAAGGAGAAGAGCCTTCAGAAGGAAGGTCCAGTCGGTCATGCCTTTGCTCGGTTGATCCGCTCCCGCGACATTGTGGCGACAGCCCTGGGTCAACTGGGCCGAGATCCGTTGATCTTCCTGCATCCGCCAGAGGCGGGATGCGAAGAATGCGATGCCGCTCGCCAGTCGATTGGC
Protein Sequence
MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG
promoter 5
Label Gene Product Position Strand
rpsL promoter — — 3092..3394 Fwd
DNA Sequence
GCCCTGCAGGCGGAAGTCAGGTAGACACGACTTCCGCTAGTCCTTGCAAGGTCTGCTGACGTGAGGCGGGGCGGTCGTTTTTGACCGCCCTGCCTTCGTCATGTAGGCTCGCTCGCTGTGCCTGGCGTGTCATCAGACGCCCAGGTCCCGGTGCCGTGAGGCCCGGGCCATCGAGCCGGTGGTACGTGGCTGCGGTCCCCTTGTGAGGGCTGCGCGCCGTGTGCTGTCCGGCGCGCACAGCCTTGAATCCACCCGCGGGGGCCGGCCGGTCTCCGTGAGCTCGAGTAGACGACGGAGACGTA
gapdh promoter — — 7700..7985 Fwd
DNA Sequence
GCTGCTCCTTCGGTCGGACGTGCGTCTACGGGCACCTTACCGCAGCCGTCGGCTGTGCGACACGGACGGATCGGGCGAACTGGCCGATGCTGGGAGAAGCGCGCTGCTGTACGGCGCGCACCGGGTGCGGAGCCCCTCGGCGAGCGGTGTGAAACTTCTGTGAATGGCCTGTTCGGTTGCTTTTTTTATACGGCTGCCAGATAAGGCTTGCAGCATCTGGGCGGCTACCGCTATGATCGGGGCGTTCCTGCAATTCTTAGTGCGAGTATCTGAAAGGGGATACGC
T7 promoter — — 8180..8199 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T3 promoter — — 8246..8265 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 8333..8364 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 8309..8326 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 8378..8400 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 8615..9204 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGGTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 8539..8574 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT