pCRISPomyces-1

61736 Synthetic Bacterial Expression

Plasmid Information

Purpose
Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
Depositor
Huimin Zhao
Article
Cobb et al ACS Synth Biol. 2014 Dec 8.
Mutation
BbsI-flanked lacZ cassette inserted in place of s…
Promoter
gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
Available Since
Jan. 27, 2015
Availability
Available

Sequence Information

Length
11,473 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 380..4487 Fwd
DNA Sequence
ATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGACTGA
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 380..4484 Fwd
DNA Sequence
ATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
lacZ-alpha lacZ fragment LacZ-alpha fragment of beta-galactosida… 5511..5808 Rev
DNA Sequence
ATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCTGA
Protein Sequence
MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG
ApmR aac(3)IV aminoglycoside 3-N-acetyltransferase ty… 6872..7673 Fwd
DNA Sequence
ATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCTTCTCAACCTTGGGGTTACCCCCGGCGGTGTGCTGCTGGTCCACAGCTCCTTCCGTAGCGTCCGGCCCCTCGAAGATGGGCCACTTGGACTGATCGAGGCCCTGCGTGCTGCGCTGGGTCCGGGAGGGACGCTCGTCATGCCCTCGTGGTCAGGTCTGGACGACGAGCCGTTCGATCCTGCCACGTCGCCCGTTACACCGGACCTTGGAGTTGTCTCTGACACATTCTGGCGCCTGCCAAATGTAAAGCGCAGCGCCCATCCATTTGCCTTTGCGGCAGCGGGGCCACAGGCAGAGCAGATCATCTCTGATCCATTGCCCCTGCCACCTCACTCGCCTGCAAGCCCGGTCGCCCGTGTCCATGAACTCGATGGGCAGGTACTTCTCCTCGGCGTGGGACACGATGCCAACACGACGCTGCATCTTGCCGAGTTGATGGCAAAGGTTCCCTATGGGGTGCCGAGACACTGCACCATTCTTCAGGATGGCAAGTTGGTACGCGTCGATTATCTCGAGAATGACCACTGCTGTGAGCGCTTTGCCTTGGCGGACAGGTGGCTCAAGGAGAAGAGCCTTCAGAAGGAAGGTCCAGTCGGTCATGCCTTTGCTCGGTTGATCCGCTCCCGCGACATTGTGGCGACAGCCCTGGGTCAACTGGGCCGAGATCCGTTGATCTTCCTGCATCCGCCAGAGGCGGGATGCGAAGAATGCGATGCCGCTCGCCAGTCGATTGGC
Protein Sequence
MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG
pSG5 Rep rep replication initiator protein from the … 8611..10054 Rev
DNA Sequence
ATGTGGAAGCCGGGCGAGGCGACGTGGGGCAACACGTGCCGCTGCAACAACGTCCACACCTGCCCGTGGTGCATGAGCCGCATCCTGGCCGTACGGGGCTCGAACGTGCAGCTCGCCGCCGACGGTCTCGCGGACGCCGGGTACGGGCTGCACCTTGGCACGAACACGTTGCGCCACTTCGAGCGCATGGCGTTCGGCACCGTCCGCAAGGGAATGCGGCACGGCCTGGTCGCGGTCTTGCACGACGGCTGGAAGGGCGCGTACGGCTCGTCCGGCCGCCGCTGGCGCACGATGCGCGACGACTTCGGGATCATCGGCTACGAGCGGGCCTTCGAGGACACCTTCGGCTGGGGCTCCGGCTGGCACCTGCACTGGCACACGCTCTGGGTCACCCGCGAGGTGCTCGGCCCGGACGCCCAGGCCGCGTTCCGCGACGCACTCGCCGGAGCCTGGGCCGCCGGGGTCGAATCCGCGGGCGGCTACACCGTCAGCGAGACGTGCGACCGGCCCGGTTGCTCCTGTGAGGGCAAGGGCCACGGCACCGACGTGCGCCCACTCAACGGCGCGGACGCCGCTGACGGCGATGCGGGCAAGCAGGCCCGCTACCTCTACAAGGACGGCGACAAGACCAAGGGCGGCGTCGCCAAGATCGGCCTGGAGCTGGCCGGACAGAACTTCAAGGCCGGTCGCGGCGACGACCGCATGGGACCGCTCGACCTGGGCGACGCGGCGGCCGCCGAGCTGCAGCGGCTTCGTCGGCCCGGCCCGTTCGTCGAGAAGTACCGCGAGCGCGAGTTCGGCGTGTTCCAGGTCCGCAAGCACTACCGGTCGCAGAACCTGAACCGGCTGATCAAGGAACTCGGCATCCAGCAGGACGTCCGCACCGAGGAGGAGATCACCGACGACACCGAGGGCCTGGTCGCCATCGCGGTCATCCCGGCCTACATCTGGTACCGATACATCGCCCGCGTCGCCGGACGTCGCCTCGACCTCATCAAGGTCGCCGAGACGTACGGCCTACCGGGCGTCCGGCGGCTCGTCGAGTCCTGGGGCCTGGTGTGGGGCAAGGACGTCCTGGACCCGCCCGCCCCGGAAGCGCCGGCCGCGCCGGGCGACCTTGACGCTGACCAGATGAGGTTCGAGGTCATGTCCGAGGAGGAAGCTGCTTTCCGCGAGGCGCGCCGGAAGGCCAACGAGGCCCGCACGGAGGAGCTGGCCGCTTCGCTCGACCGAGTGCGTCAGCCGAAGAAGGAGGCGATCCGCCCGACGATCAGCCTTCGGAAGCGCCTGAAGCCGAAGCCGGTCACCGTCGACGTCAAGACTCCCCCGCCCGGCGCCGCGTCGCCCGTGTGCCGCCGCTGCAAGGGCAAGCTCGCCCCGGTACTCCAGCCCTGGGGCCAGCACCCCGGAGACTGCCTCCGCGTCGACACAGCGGTCGCGTAG
Protein Sequence
MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLADAGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGIIGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTVSETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELAGQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLNRLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGLPGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANEARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKLAPVLQPWGQHPGDCLRVDTAVA
promoter 6
Label Gene Product Position Strand
rpsL promoter — — 78..380 Fwd
DNA Sequence
GCCCTGCAGGCGGAAGTCAGGTAGACACGACTTCCGCTAGTCCTTGCAAGGTCTGCTGACGTGAGGCGGGGCGGTCGTTTTTGACCGCCCTGCCTTCGTCATGTAGGCTCGCTCGCTGTGCCTGGCGTGTCATCAGACGCCCAGGTCCCGGTGCCGTGAGGCCCGGGCCATCGAGCCGGTGGTACGTGGCTGCGGTCCCCTTGTGAGGGCTGCGCGCCGTGTGCTGTCCGGCGCGCACAGCCTTGAATCCACCCGCGGGGGCCGGCCGGTCTCCGTGAGCTCGAGTAGACGACGGAGACGTA
rpsL promoter — — 4686..5047 Fwd
DNA Sequence
CCGCCCGCGGGACGTGACGAGCGGCACGACTCGACGACTCCGGGCTCCTTTGACGCTGTCCGTCGCGCCGGGTAGCGTAGGACACCGTGCCCGCGCCGTCGGGCCCTCGCGCGTGCACTCGGTCGACCGCTCCCTGCCGGAGTGGGTGCGGGTGCACGGGGTGGCTCCCCACCTCCTCTCGGATCGGTCCTCGCGGACTGCCGCCGTGCGGAGGACCGGGGCGACACGCCCGGGCGCGGGGGTCGGTGCGGGACTCCAGACCTCCGGGGTAGTCGTGCGACGGGCGACGATCCGGGCCGAGCCGGCCGTCCTGGGTGACGGGTGCCGGTCAGACCAGAGAACACCGACAGACGGAGACGTA
gapdh promoter — — 5183..5468 Fwd
DNA Sequence
GCTGCTCCTTCGGTCGGACGTGCGTCTACGGGCACCTTACCGCAGCCGTCGGCTGTGCGACACGGACGGATCGGGCGAACTGGCCGATGCTGGGAGAAGCGCGCTGCTGTACGGCGCGCACCGGGTGCGGAGCCCCTCGGCGAGCGGTGTGAAACTTCTGTGAATGGCCTGTTCGGTTGCTTTTTTTATACGGCTGCCAGATAAGGCTTGCAGCATCTGGGCGGCTACCGCTATGATCGGGGCGTTCCTGCAATTCTTAGTGCGAGTATCTGAAAGGGGATACGC
T7 promoter — — 5698..5717 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T3 promoter — — 5764..5783 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 5851..5882 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 5827..5844 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 5896..5918 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 6080..6669 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGGTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
lambda t0 terminator — — 5149..5183 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTA
T7 terminator — — 5990..6038 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG