Human_TFAM_NoMTS_L6_pET28a+

60012 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses TFAM No MTS L6 mutant in bacterial cells
Depositor
David Chan
Article
Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc
Insert
Human TFAM No MTS (TFAM Human)
Tags
6xHis
Mutation
Missing 1st 42 aas (Mitochondrial targeting seque…
Promoter
T7
Available Since
Oct. 21, 2014
Availability
Available

Sequence Information

Length
733 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 484..502 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 511..529 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 532..565 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
promoter 2
Label Gene Product Position Strand
lacI promoter lacI — 0..77 Rev
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAA
T7 promoter — — 385..404 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 404..429 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC