pRSETC PITPbeta N89F

58279 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PITP beta N89F mutant
Depositor
Shamshad Cockcroft
Article
Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7
Insert
PITP beta (PITPNB Human)
Tags
HIS
Mutation
N89F (abolishes PI binding)
Promoter
T7
Available Since
July 21, 2014
Availability
Available

Sequence Information

Length
758 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 42..60 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 63..96 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 99..123 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK