pRSETC-PITPbeta (splice variant 1) K61A

58271 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)
Depositor
Shamshad Cockcroft
Article
Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10
Insert
PITP beta (PITPNB Human)
Tags
HIS
Mutation
K61A (abolishes PI binding)
Promoter
T7
Available Since
July 15, 2014
Availability
Available

Sequence Information

Length
843 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 61..94 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 97..121 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK