pAtCHYb1Δ129N

53367 Arabidopsis thaliana Bacterial Expression

Plasmid Information

Purpose
Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.
Depositor
Francis X Cunningham Jr
Article
Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.
Insert
chyB1 (BETA-OHASE 1 Mustard Weed)
Mutation
Deleted codons for the first 129 amino acids
Promoter
Trc
Available Since
April 16, 2015
Availability
Available

Sequence Information

Length
811 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 2..20 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 23..56 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 59..83 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK