pTrcHisB-TRF1

53209 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expressed N-terminally Hexahistidine tagged TRF1 protein
Depositor
Jack Griffith
Article
Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.
Insert
TRF1 (TERF1 Human)
Tags
Hexahistidine
Mutation
3-439
Promoter
Trc
Available Since
May 20, 2014
Availability
Available

Sequence Information

Length
913 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
minicistron synthetic cistron containing a ribosome… 98..125 Fwd
DNA Sequence
ATGTATCGATTAAATAAGGAGGAATAA
Protein Sequence
MYRLNKEE
6xHis 6xHis affinity tag 140..158 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 161..194 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
TEV site tobacco etch virus (TEV) protease recog… 206..227 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG