Human HMG-CoA lyase (HL)

52433 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of human HMG-CoA lyase (HL)
Depositor
Henry Miziorko
Article
Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):
Insert
HMG CoA lyase (HMGCL Human)
Tags
His and S
Promoter
T7
Available Since
April 8, 2014
Availability
Available

Sequence Information

Length
892 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 137..155 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 164..182 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag — affinity and epitope tag derived from p… 188..233 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site — enterokinase recognition and cleavage s… 248..263 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
promoter 1
Label Gene Product Position Strand
T7 promoter — — 46..65 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 65..90 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC