pET-Trx-mSA2

52320 Synthetic Bacterial Expression

Plasmid Information

Purpose
Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
Depositor
Sheldon Park
Article
Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
Insert
monomeric streptavidin 2
Tags
FLAG, His, S tag, TEV site, and Thioredoxin
Mutation
S25H mutation compared to streptavidin
Promoter
T7
Available Since
July 15, 2014
Availability
Available

Sequence Information

Length
910 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 7
Label Gene Product Position Strand
TrxA trxA E. coli thioredoxin 22..349 Fwd
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
6xHis 6xHis affinity tag 370..388 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 397..415 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 421..466 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site enterokinase recognition and cleavage s… 481..496 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
TEV site tobacco etch virus (TEV) protease recog… 529..550 Fwd
DNA Sequence
GAGAACCTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
mSA monomeric streptavidin/rhizavidin hybri… 550..892 Fwd
DNA Sequence
GCGGAAGCGGGTATCACCGGCACGTGGTACAACCAGCATGGTTCTACCTTCACCGTTACCGCGGGTGCGGACGGTAACCTGACCGGTCAGTACGAAAACCGTGCGCAGGGCACTGGTTGCCAGAACTCTCCGTACACCCTGACCGGTCGTTACAACGGTACCAAACTGGAATGGCGTGTTGAATGGAACAACTCTACCGAAAACTGCCACTCTCGTACCGAATGGCGTGGTCAGTACCAGGGTGGTGCGGAAGCGCGTATCAACACCCAGTGGAACCTGACCTACGAAGGTGGTTCTGGTCCGGCGACCGAACAGGGTCAGGACACCTTCACCAAAGTTAAA
Protein Sequence
AEAGITGTWYNQHGSTFTVTAGADGNLTGQYENRAQGTGCQNSPYTLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATEQGQDTFTKVK