His-MBP human SPOP (aa 28-337)

52294 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of human SPOP (aa 28-337) fused to His-MBP
Depositor
Brenda Schulman
Article
Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
Insert
SPOP (SPOP Human)
Tags
His-MBP and TEV
Promoter
T7
Available Since
April 11, 2014
Availability
Available

Sequence Information

Length
785 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
S-Tag — affinity and epitope tag derived from p… 5..50 Rev
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 183..202 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 158..183 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC