Nano-lantern(Ca2+)_63/pRSETB

51980 Synthetic Bacterial Expression

Plasmid Information

Purpose
bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM
Depositor
Takeharu Nagai
Article
Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc
Insert
Nano-lantern_CaM_E104Q-4GS
Tags
6xHis
Mutation
S257G in Rluc8
Available Since
March 25, 2014
Availability
Available

Sequence Information

Length
860 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 38..56 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 59..92 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 95..119 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
VN173 N-terminal fragment of mVenus for use i… 125..644 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE