pET21a-hAIM2-deltaPYD

51522 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Express His6-tagged HIN domain of hAIM2 in bacteria
Depositor
Emad Alnemri
Article
Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do
Insert
AIM2 (AIM2 Human)
Tags
His6 and T7
Mutation
deletion of pyrin domain
Promoter
lac operator
Available Since
March 10, 2014
Availability
Available

Sequence Information

Length
977 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 44..77 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
6xHis — 6xHis affinity tag 824..842 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 908..956 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG