pTRCHisB-TRF2_M

50490 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
E. Coli N-term HexHis Tagged Protein Expression Vector
Depositor
Jack Griffith
Article
Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.
Insert
TRF2 Myb (TERF2 Human)
Tags
6x His, T7 tag, and TEV protease site
Mutation
Contains amino acids 438-500
Promoter
pTrc
Available Since
Feb. 3, 2014
Availability
Available

Sequence Information

Length
790 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
minicistron synthetic cistron containing a ribosome… 106..133 Fwd
DNA Sequence
ATGTATCGATTAAATAAGGAGGAATAA
Protein Sequence
MYRLNKEE
6xHis 6xHis affinity tag 148..166 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 169..202 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
TEV site tobacco etch virus (TEV) protease recog… 214..235 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 668..755 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT