pFA3

46790 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
To express ATPase inactive Fun30 in budding yeast
Depositor
Patrick Varga-Weisz
Article
Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1
Insert
FUN30 (FUN30 Budding Yeast)
Tags
V5
Mutation
ATP-binding site mutated replaced lysin 603 by ar…
Promoter
PGAL1
Available Since
Aug. 7, 2013
Availability
Available

Sequence Information

Length
921 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 19..38 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
ZF binding site — — 90..102 Fwd
DNA Sequence
GATGAGGATGAC