pET29b_Qcat11

45139 Synthetic Bacterial Expression

Plasmid Information

Depositor
Christopher Petzold
Article
Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1
Insert
E-coli-pathways-QconCAT11
Tags
6XHis
Promoter
T7
Available Since
Dec. 12, 2013
Availability
Available

Sequence Information

Length
903 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
S-Tag affinity and epitope tag derived from p… 138..183 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
thrombin site thrombin recognition and cleavage site 198..216 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter 47..66 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 66..91 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC