| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| S-Tag | — | affinity and epitope tag derived from p… | 147..192 | Fwd | |
|
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
|
|||||
| thrombin site | — | thrombin recognition and cleavage site | 207..225 | Fwd | |
|
DNA Sequence
CTGGTGCCACGCGGTTCC
Protein Sequence
LVPRGS
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 56..75 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 75..100 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||