pET29b_Qcat4

45132 Synthetic Bacterial Expression

Plasmid Information

Depositor
Christopher Petzold
Article
Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1
Insert
E-coli-pathways-QconCAT4
Tags
6XHis
Promoter
T7
Available Since
Dec. 12, 2013
Availability
Available

Sequence Information

Length
847 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
S-Tag affinity and epitope tag derived from p… 137..182 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
thrombin site thrombin recognition and cleavage site 197..215 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter 46..65 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 65..90 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC