His-NKX3.1

42896 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Edward Gelmann
Article
Ju et al Biochemistry. 2009 Nov 10;48(44):10601-7
Insert
NKX3.1 (NKX3-1 Human)
Tags
6xHis
Available Since
April 9, 2013
Availability
Available

Sequence Information

Length
794 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 144..162 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 171..189 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 195..240 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site enterokinase recognition and cleavage s… 255..270 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
promoter 1
Label Gene Product Position Strand
T7 promoter 53..72 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 72..97 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC