pRSET-RCaMP1h

42874 Synthetic Bacterial Expression

Plasmid Information

Purpose
a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
Depositor
Loren Looger
Article
Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
Insert
Red Fluorescent Calcium binding Protein
Tags
EK (Enterokinase) Recognition Site, His Tag (6x),…
Promoter
T7
Available Since
Feb. 22, 2013
Availability
Available

Sequence Information

Length
1,050 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 53..71 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 74..107 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 110..134 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
calmodulin-binding peptide calmodulin-binding peptide from avian s… 161..218 Fwd
DNA Sequence
CGTCGTAAGTGGAATAAGACAGGTCACGCAGTCAGAGCTATAGGTCGGCTGAGCTCA
Protein Sequence
RRKWNKTGHAVRAIGRLSS