pcold-6xHis-HRV3Csite-DmLoqsPD

41095 Drosophila melanogaster Bacterial Expression

Plasmid Information

Depositor
Phillip Zamore
Article
Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011
Insert
Loquacious-PD (loqs Fly)
Tags
6xHis-HRV3Csite
Available Since
Dec. 3, 2012
Availability
Available

Sequence Information

Length
879 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
TEE cspA translation enhancing element for E. co… 2..17 Fwd
DNA Sequence
ATGAATCACAAAGTG
Protein Sequence
MNHKV
6xHis 6xHis affinity tag 17..35 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
Factor Xa site Factor Xa recognition and cleavage site 35..47 Fwd
DNA Sequence
ATCGAAGGTAGG
Protein Sequence
IEGR
HRV 3C site recognition and cleavage site for human… 83..107 Fwd
DNA Sequence
CTTGAAGTCCTCTTTCAGGGACCC
Protein Sequence
LEVLFQGP