LHP2691 - pET30-Ub-K63A

32179 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Linda Hicke
Insert
synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
Tags
HIS
Mutation
K63A
Promoter
T7
Available Since
Sept. 27, 2011
Availability
Available

Sequence Information

Length
820 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 6
Label Gene Product Position Strand
6xHis 6xHis affinity tag 31..49 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 58..76 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 82..127 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
Factor Xa site Factor Xa recognition and cleavage site 157..169 Fwd
DNA Sequence
ATTGAGGGTCGC
Protein Sequence
IEGR
ubiquitin Homo sapiens UBC human ubiquitin 169..397 Fwd
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAAGCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGGCGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQAESTLHLVLRLRGG
6xHis 6xHis affinity tag 478..496 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator 562..610 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG