GST-TEV-FUS-1-413aa

29632 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Aaron Gitler
Article
Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:
Insert
FUS-1-413aa (FUS Human)
Tags
GST and TEV
Mutation
Contains FUS aa# 1-413
Available Since
July 18, 2011
Availability
Available

Sequence Information

Length
896 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
S-Tag — affinity and epitope tag derived from p… 15..60 Rev
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 193..212 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 168..193 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC
attB2 mutant version of attB — 323..348 Fwd
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT