GST-TEV-FUS-1-373aa

29631 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Aaron Gitler
Article
Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:
Insert
FUS-1-373aa (FUS Human)
Tags
GST and TEV
Mutation
Contains FUS aa# 1-373
Available Since
June 6, 2011
Availability
Available

Sequence Information

Length
890 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
S-Tag — affinity and epitope tag derived from p… 10..55 Rev
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 188..207 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 163..188 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC
attB2 mutant version of attB — 318..343 Fwd
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT