pDuet TDP-43 G294A — PlasmidDB

Plasmid Information

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 10, 2011

Availability

Available

Length

891 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
S-Tag	—	affinity and epitope tag derived from p…	14..59	Rev
DNA Sequence AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG Protein Sequence KETAAAKFERQHMDS

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	192..211	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	167..192	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC