Ubp8/PET32a

26965 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Cynthia Wolberger
Article
Samara et al Science. 2010 May 21. 328(5981):1025-9.
Insert
Ubp8 (UBP8 Budding Yeast)
Tags
His6 and Trx
Mutation
TEV site is added t the 5' end and 4 alanine…
Available Since
Jan. 25, 2011
Availability
Available

Sequence Information

Length
898 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 6
Label Gene Product Position Strand
TrxA trxA E. coli thioredoxin 46..373 Fwd
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
6xHis 6xHis affinity tag 394..412 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 421..439 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 445..490 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site enterokinase recognition and cleavage s… 505..520 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
TEV site tobacco etch virus (TEV) protease recog… 562..583 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG