Sus1/PET32a

26963 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Cynthia Wolberger
Article
Samara et al Science. 2010 May 21. 328(5981):1025-9.
Insert
Sus1 (SUS1 Budding Yeast)
Tags
His6 and Trx
Mutation
with TEV site to cleave the N-terminal his+Trx tag
Available Since
Jan. 5, 2011
Availability
Available

Sequence Information

Length
942 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 7
Label Gene Product Position Strand
6xHis 6xHis affinity tag 29..47 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
TEV site tobacco etch virus (TEV) protease recog… 353..374 Rev
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG
enterokinase site enterokinase recognition and cleavage s… 383..398 Rev
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
S-Tag affinity and epitope tag derived from p… 413..458 Rev
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
thrombin site thrombin recognition and cleavage site 464..482 Rev
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 491..509 Rev
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TrxA trxA E. coli thioredoxin 530..857 Rev
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
protein_bind 1
Label Gene Product Position Strand
lac operator 901..926 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC