pET-30 Ubwt (2)

26685 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Linda Hicke
Article
French et al J Biol Chem. 2009 May 1. 284(18):12071-9
Insert
Ubiquitin
Tags
His and S-tag
Available Since
Nov. 17, 2010
Availability
Available

Sequence Information

Length
797 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 6
Label Gene Product Position Strand
6xHis 6xHis affinity tag 51..69 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 78..96 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 102..147 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
Factor Xa site Factor Xa recognition and cleavage site 177..189 Fwd
DNA Sequence
ATTGAGGGTCGC
Protein Sequence
IEGR
ubiquitin Homo sapiens UBC human ubiquitin 189..417 Fwd
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAAGCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
6xHis 6xHis affinity tag 498..516 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator 582..630 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG