| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 56..74 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
|
|||||
| thrombin site | — | thrombin recognition and cleavage site | 83..101 | Fwd | |
|
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
|
|||||
| 6xHis | — | 6xHis affinity tag | 304..322 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| M13 ori | — | — | 472..853 | Fwd | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 388..436 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||