pLlacO1-DP6

242138 Synthetic Bacterial Expression

Plasmid Information

Purpose
AraC compatible Mutagenesis Circuit (drift plasmid)
Depositor
Dominik Niopek
Article
Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:
Insert
Gene III
Available Since
Sept. 19, 2025
Availability
Available

Sequence Information

Length
7,563 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
CmR cat chloramphenicol acetyltransferase 114..774 Fwd
DNA Sequence
ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTAAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAGTTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACTATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA
Protein Sequence
MEKKITGYTTVDISQWHRKEHFEAFQSVAKCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA
PmCDA1 — cytidine deaminase from the sea lamprey… 847..1474 Rev
DNA Sequence
ATGACCGACGCGGAATACGTTCGTATCCACGAAAAACTGGACATCTACACCTTCAAAAAACAGTTCTTCAACAACAAAAAATCTGTTTCTCACCGTTGCTACGTTCTGTTCGAACTGAAACGTCGTGGTGAACGTCGTGCGTGCTTCTGGGGTTACGCGGTTAACAAACCGCAGTCTGGTACCGAACGTGGTATCCACGCGGAAATCTTCTCTATCCGTAAAGTTGAAGAATACCTGCGTGACAACCCGGGTCAGTTCACCATCAACTGGTACTCTTCTTGGTCTCCGTGCGCGGACTGCGCGGAAAAAATCCTGGAATGGTACAACCAGGAACTGCGTGGTAACGGTCACACCCTGAAAATCTGGGCGTGCAAACTGTACTACGAAAAAAACGCGCGTAACCAGATCGGTCTGTGGAACCTGCGTGACAACGGTGTTGGTCTGAACGTTATGGTTTCTGAACACTACCAGTGCTGCCGTAAAATCTTCATCCAGTCTTCTCACAACCAGCTGAACGAAAACCGTTGGCTGGAAAAAACCCTGAAACGTGCGGAAAAACGTCGTTCTGAACTGTCTATCATGATCCAGGTTAAAATCCTGCACACCACCAAATCTCCGGCGGTTTAA
Protein Sequence
MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGERRACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKILEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIFIQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV
UGI — uracil-DNA glycosylase inhibitor from a… 1503..1758 Rev
DNA Sequence
ATGACAAATTTATCTGACATCATTGAAAAAGAAACAGGAAAACAACTAGTGATTCAAGAATCAATTCTAATGTTACCAGAAGAAGTAGAGGAAGTAATTGGGAATAAACCAGAAAGTGATATTTTAGTTCATACTGCTTATGATGAAAGTACAGATGAAAATGTAATGCTATTAACTTCAGATGCTCCAGAATATAAACCTTGGGCTTTAGTAATTCAAGACAGTAATGGAGAAAATAAAATTAAAATGTTATAA
Protein Sequence
MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
protein_bind 1
Label Gene Product Position Strand
lac operator — — 4573..4590 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
terminator 3
Label Gene Product Position Strand
tonB terminator — — 789..821 Rev
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGACT
rrnB T1 terminator Escherichia coli rrnB — 4833..4920 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT
rrnB T2 terminator — — 5011..5039 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT