pWN062

233822 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
MoClo-YTK; type3A; NS3 systems; LexA-NES
Depositor
Jeff Hasty
Article
O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
Insert
LexA-NES
Available Since
May 22, 2025
Availability
Available

Sequence Information

Length
2,313 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
LexA lexA E. coli DNA-binding protein that repres… 10..616 Fwd
DNA Sequence
ATGAAGGCACTAACGGCGCGTCAACAGGAAGTATTTGATCTAATCAGAGATCACATTTCTCAGACGGGCATGCCACCTACGAGAGCGGAAATTGCGCAGAGGCTGGGGTTTCGTTCACCAAACGCGGCCGAGGAACACCTGAAGGCTCTAGCAAGAAAAGGTGTCATTGAGATAGTCTCCGGTGCCTCCAGGGGAATCAGGCTTCTACAAGAGGAGGAGGAAGGACTGCCACTTGTAGGAAGGGTCGCTGCAGGGGAGCCTCTATTGGCGCAGCAGCATATCGAAGGTCATTATCAGGTCGATCCATCTTTGTTCAAGCCCAATGCCGATTTCTTACTTCGTGTGAGTGGAATGAGTATGAAAGATATAGGCATTATGGATGGGGATCTACTTGCTGTTCATAAAACACAAGATGTGAGAAATGGTCAGGTAGTTGTAGCAAGGATCGATGACGAAGTCACAGTTAAAAGGCTAAAGAAGCAAGGCAATAAAGTAGAGTTATTACCCGAGAACTCAGAATTTAAGCCTATTGTGGTCGACCTACGTCAACAGAGCTTCACCATAGAAGGTCTGGCCGTCGGTGTTATCCGTAACGGTGATTGGCTG
Protein Sequence
MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL
NES nuclear export signal from the HIV Rev … 631..658 Fwd
DNA Sequence
CTGCCTCCGTTAGAAAGACTAACGCTG
Protein Sequence
LPPLERLTL
CmR cat chloramphenicol acetyltransferase 782..1442 Rev
DNA Sequence
ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTTTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA
Protein Sequence
MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA
rep_origin 1
Label Gene Product Position Strand
ori 1623..2212 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator 677..712 Rev
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT