pRO112

233806 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
MoClo-YTK; type3; rtTA systems; rtTA-v10
Depositor
Jeff Hasty
Article
O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
Insert
rtTA-v10
Available Since
May 22, 2025
Availability
Available

Sequence Information

Length
2,409 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
Tet-On(R) 3G modified rtTA protein that binds tightl… 366..1110 Fwd
DNA Sequence
ATGTCCCGTCTTGATAAGAGTAAGGTTATCAACTCAGCCCTAGAGCTGCTGAACGGCGTCGGAATCGAGGGGCTTACTACCAGAAAACTAGCACAGAAACTGGGTGTAGAGCAGCCCACGCTTTACTGGCATGTTAAAAATAAGAGAGCACTATTAGACGCGTTGCCGATCGAAATGTTGGATAGGCATCACACACACTCATGCCCTTTAGAAGGAGAAAGTTGGCAGGATTTTCTGAGAAATAATGCGAAGTCTTATCGTTGTGCGTTACTTTCCCACAGGGACGGTGCTAAAGTACATTTGGGAACTAGACCAACTGAAAAACAATATGAGACATTGGAGAACCAACTGGCCTTCTTATGCCAACAGGGTTTCAGTCTTGAGAACGCCCTTTACGCCCTTTCAGCGGTTGGGCACTTCACTCTTGGTTGCGTCTTGGAAGAGCAGGAGCATCAAGTAGCAAAAGAGGAGCGTGAAACCCCCACAACAGACTCCATGCCCCCTCTATTGAAGCAAGCTATCGAATTATTCGACCGTCAAGGAGCGGAACCAGCATTTTTATTTGGCTTAGAATTGATTATTTGTGGGTTAGAAAAACAACTAAAGTGTGAGAGCGGTGGACCGACAGACGCTCTTGATGATTTTGACTTGGATATGTTACCCGCCGACGCTTTGGACGACTTCGATTTGGATATGCTGCCTGCGGATGCACTAGATGATTTCGATCTGGACATGTTACCGGGT
Protein Sequence
MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG
CmR cat chloramphenicol acetyltransferase 1234..1894 Rev
DNA Sequence
ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTTTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA
Protein Sequence
MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA
rep_origin 1
Label Gene Product Position Strand
ori 0..2409 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator 1129..1164 Rev
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT